摘要 |
A method for modeling the intestinal ischemic-reperfusion injury comprises the anesthesia, the opening of the abdominal cavity by the layer-by-layer median laparotomy, the separation of the trunk of the superior mesenteric artery, and the temporary ischemization of the blood-supplied region of the intestine. The intestine is exteriorized to the laparotomic wound and ischemized in the area in length. Through the mesenteric edge of the intestine, the ligature is applied near the branching of the superior mesenteric artery, and the ends of the ligature are tied up with the provisional knot. The intestine is then descended into the abdominal cavity. The provisional knot of ligature is exteriorized through the laparotomic wound and is separated. The ligature is removed in 30 minutes. Anesthesia is performed by intraperitoneal injection. |