TUMOR TISSUE MICROARRAYS FOR RAPID MOLECULAR PROFILING

摘要

<P>PROBLEM TO BE SOLVED: To provide a method of conducting rapid parallel analysis of molecular characteristics of hundreds of tumor specimens, which are morphologically controlled, capable of performing large-scale molecular profiling of tissue specimens with a minimum tissue requirement. Ž<P>SOLUTION: An array-based technology facilitates rapid correlated gene copy number and expression profiling of a very large numbers of human tumors. Hundreds of cylindrical tissue biopsies (a diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block. Consecutive sections from such arrays provide targets for parallel in situ hybridization and quantification of DNA, RNA or protein targets. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž