ANTISENSE INHIBITION OF LAMININ-8 EXPRESSION TO INHIBIT HUMAN GLIOMAS

摘要

PROBLEM TO BE SOLVED: To provide antisense oligonucleotides which bind a specific RNA arrangement and also inactivate this.

SOLUTION: Using gene array technology, it is observed that an increase in α4 chain-containing Laminin-8 associated with poor prognosis for patients with brain gliomas. It is established that inhibition of Laminin-8 expression by new generation of highly specific and stable antisense oligonucleotides (MorpholinoTM) against chains of Laminin-8 can slow or stop the spread of glioma. This has been demonstrated in an in vitro model using human glioblastoma multiforme cell lines M059K and U-87MG co-cultured with normal human brain microvascular endothelial cells (HBMVEC). Using Western blot analysis and immunohistochemistry, it has been confirmed that antisense treatment effectively blocks Laminin-8 protein synthesis.

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