发明名称 |
BIOSENSING METHOD FOR TARGET DNA USING MEDIATOR-MODIFIED PRIMER |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide a biosensing method for detecting a target DNA at low cost simply in high sensitivity. <P>SOLUTION: The biosensing method for detecting a target DNA comprises: modifying a PCR primer of the target DNA with a mediator for transferring electrons caused by a reaction between an enzyme and a substrate in an electrolyte to which an electric current is applied; subjecting the target DNA to PCR amplification using the mediator-modified primer; adding a PCR product and a substrate to an electrolyte equipped with an enzyme immobilized electrode, applying an electric current to the electrolyte; measuring an electric signal from the enzyme immobilized electrode and, when an electric current is detected; and determining that the target DNA exists in an analysis sample. <P>COPYRIGHT: (C)2010,JPO&INPIT |
申请公布号 |
JP2009240197(A) |
申请公布日期 |
2009.10.22 |
申请号 |
JP20080089444 |
申请日期 |
2008.03.31 |
申请人 |
UNIV OF FUKUI;ARCADIA SYSTEMS INC |
发明人 |
SUE SHINICHIRO;KOBAYASHI TAKESHI |
分类号 |
C12Q1/68;C12N15/09;G01N27/327;G01N27/416 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|