MESENCHYMAL STEM CELL AND METHOD FOR PRODUCTION THEREOF

摘要

Disclosed is a method for producing a mesenchymal stem cell capable of being differentiated into a myoblast by culturing a pluripotent stem cell derived from a human body or an animal. The method comprises the following steps i) to iv): i) providing the pluripotent stem cell which has been cryopreserved; ii) subculturing the pluripotent stem cell for a predetermined number of times while keeping the pluripotent stem cell in an undifferentiated state; iii) culturing the subcultured pluripotent stem cell under conditions which enable the induction of the differentiation of the pluripotent stem cell into a fat cell in vitro; and iv) separating and collecting a CD105-positive cell in the culturing step.