METHOD FOR QUANTIFICATION OF G-AMINOBUTYRIC ACID

摘要

Disclosed is a method for quantifying ?-aminobutyric acid, which comprises the steps of: producing reduced nicotinamide adenine dinucleotidephosphate by using a specific aminotransferase and a dehydrogenase that needs to use oxidized nicotinamide adenine dinucleotidephosphate as a coenzyme and deactivating the enzymes, thereby removing any amino acid having a similar structure to that of GABA (e.g., glutamic acid); and acting an electron carrier on NADPH produced in the aforementioned step in the presence of a tetrazolium salt that can produce a water-soluble formazan dye and measuring the water-soluble formazan dye produced.