发明名称 |
METHOD OF SEPARATING AND PURIFYING CELLULAR COMPONENTS USING NON-COVALENT BOND BETWEEN CUCURBITURIL DERIVATIVE AND GUEST COMPOUND AND APPARATUS USING THE SAME |
摘要 |
<p>Provided is a method of separating cellular components, the method including: a) contacting a guest compound-bound reactive compound with cells; b) lysing the cells; c) adding a host compound-bound solid phase to a solution including the lysates of the cells to prepare a mixture; d) separating binding pairs of the guest compound bound to cellular components and the host compound bound to the solid phase from the mixture, and purifying the binding pairs; and e) separating the cellular components from the binding pairs, wherein the guest compound-bound reactive compound is obtained through a covalent bond between a reactive compound and a guest compound represented by Formula 2 below guest, the host compound-bound solid phase is obtained through a covalent bond between a solid phase and a host compound represented by Formula 1 below, and the reactive compound includes at least one selected from the group consisting of a biomolecule, N-hydroxysuccimide, an antigen, an antibody, an aptamer, folic acid, transferrin, and any mixtures thereof.</p> |
申请公布号 |
CA2721673(A1) |
申请公布日期 |
2009.10.22 |
申请号 |
CA20092721673 |
申请日期 |
2009.04.15 |
申请人 |
POSTECH ACADEMY-INDUSTRY FOUNDATION |
发明人 |
LEE, DON-WOOK;RYU, SUNG HO;HA, SANG HOON;JUNG, HYUN TAE;MAINAK, BANERJEE;SELVAPALAM, NARAYANAN;KIM, KI MOON;PARK, KYENG-MIN |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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