| 摘要 |
<P>PROBLEM TO BE SOLVED: To realize measurement with reproducibility simply and inexpensively in fluorescent detection using surface plasmon. Ž<P>SOLUTION: In the fluorescent detection using the surface plasmon, excitation light La of a wavelength that excites a fluorescent label F and reference light Lb with a wavelength longer than that of this excitation light La are irradiated through a dielectric plate 11 toward an interface between the dielectric plate 11 and a metallic film 12, so that a first electrical field enhancing field Ewa and a second electrical field enhancing field Ewb are respectively generated on the top surface of the metallic film 12. Then, the strength of the first electrical filed enhancing field Ewa is estimated from the relation between the strength of the first electrical field enhancing field Ewa and the second electrical field enhancing field Ewb by use of the scattered light Lsb of the second electrical field enhancing field Ewb that is approximately proportional to the strength of the second electrical field enhancing field Ewb, and the fluorescence amount of fluorescence Lf emitted from the fluorescent label F is normalized to compensate it to the strength of the first electrical field enhancing field Ewa. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
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