INTRACELLULAR PRODUCTION OF HEPATITIS C E1 AND E2 TRUNCATED POLYPEPTIDE

摘要

PROBLEM TO BE SOLVED: To provide methods for obtaining C-terminally truncated E1 and E2 polypeptides from cell lysates.

SOLUTION: Provided is a method for isolating a hepatitis C virus (HCV) E1 polypeptide that lacks a portion of its C-terminus beginning at about amino acid 370 but not extending beyond about amino acid 300, numbered with reference to the HCV1 E1 amino acid sequence. The method comprises: (a) a step for providing a population of host cells transformed with a polynucleotide comprising a coding sequence for the HCV E1 polypeptide, wherein the coding sequence is operably linked to control elements such that the coding sequence can be transcribed and translated in the host cells; (b) a step for culturing the population of cells under conditions whereby the HCV E1 polypeptide is expressed intracellularly; (c) a step for disrupting the host cells; and (d) a step for isolating the HCV E1 polypeptide from the disrupted cells.

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