| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for using menstrual blood collectable at home by herself as a specimen for the PCR amplification of a DNA of Chlamydia trachomatis causing a worldwide problem as the pathogen of a sexually transmitted disease. Ž<P>SOLUTION: Provided is a method for detecting a Chlamydia DNA by PCR amplification using menstrual blood as a test specimen. Menstrual blood absorbed in a PVA sponge chip absorbent is used as a starting specimen. The menstrual blood absorbed PVA sponge chip absorbent is put into a vessel, sterilized physiological saline water is poured into the vessel and stirred. After the sponge chip absorbent is squeezed to obtain a liquid sample, a DNA sample is extracted from the liquid, and the DNA sample is subjected to PCR. Then, the PCR product is subjected to nucleic acid hybridization to determine the presence of Chlamydia DNA. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
|
