发明名称 |
APOPTOSIS METHODS, GENES AND PROTEINS |
摘要 |
A W303a Saccharomyces cerevisiae yeast cell which contains a polynucleotide that encodes a functional Bax polypeptide under the control of a galactose-inducible promoter that is integrated at the LEU2 chromosomal locus. A kit of parts comprising the yeast cells and a yeast plasmid vector suitable for transforming a cDNA library into the yeast cells. Use of the yeast cell for screening a cDNA library for a polynucleotide that is or encodes an inhibitor of Bax-mediated apoptosis. Genes and polypeptides that inhibit Bax-mediated apoptosis and which were identified from a human hippocampus cDNA library screened in the yeast cells. A method of combating Bax-mediated apoptosis in a cell using an inhibitor of Bax-mediated apoptosis which was identified from a human hippocampus cDNA library screened in the yeast cells. A method of promoting Bax-mediated apoptosis in a cell using an inhibitor or antagonist of the anti-apoptotic polypeptides identified from a human hippocampus cDNA library screened in the yeast cells.
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申请公布号 |
US2009258794(A1) |
申请公布日期 |
2009.10.15 |
申请号 |
US20070279642 |
申请日期 |
2007.02.15 |
申请人 |
CHAUDHURI BHABATOSH |
发明人 |
CHAUDHURI BHABATOSH |
分类号 |
C40B30/06;C12N1/19;C40B40/02 |
主分类号 |
C40B30/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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