发明名称 |
PEPTIDFRAGMENTATION |
摘要 |
The present invention relates to a method of identifying a polypeptide using a class of novel, water-stable reagents. More specifically, the method according to the invention comprises the steps of (a) derivatization in an aqueous solution of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; (b) analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern; and (c) interpreting the fragmentation pattern. Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique, which kit comprises at least one acidic reagent comprising a sulfonyl moiety coupled to an activated acid moiety in a container together with suitable means for analysis of the fragmentation pattern obtained. |
申请公布号 |
AT444490(T) |
申请公布日期 |
2009.10.15 |
申请号 |
AT20020739339T |
申请日期 |
2002.05.20 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
LIMINGA, MARIA;MALOISEL, JEAN-LUC;PALMGREN, RONNIE;KEOUGH, THOMAS;YOUNGQUIST, ROBERT S.;VAUGHN, HAROLD;YELM, KEN |
分类号 |
G01N33/58;C07D207/48;C12Q1/37;G01N33/68 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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