| 摘要 |
A microscope has an oil immersion system objective and an imaging lens to make observations by charging oil between a cover glass protecting a sample and the most sample-side lens surface. At least one of a plurality of lens units, placed on the image side of the most sample-side lens in the oil immersion system objective, is moved along the optical axis, and thereby a front focal distance and a front focal position can be adjusted so that a predetermined depth position of the sample is focused in a state where a working distance WD between the cover glass and the most sample-side lens surface in the oil immersion system objective is set to a condition, 0<WD<=0.03 mm.
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