METHOD AND DEVICE FOR HIGH SPEED QUANTITATIVE MEASUREMENT OF BIOMOLECULAR TARGETS ON OR IN BIOLOGICAL ANALYSIS MEDIUM

摘要

The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following steps: a) at least two laser beams (F") are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and analyzed for each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the quantified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations of the latter in mixtures of the quantified element and the exogenous element in known proportions.