SELF-CALIBRATING GRADIENT DILUTION IN A CONSTITUENT ASSAY AND GRADIENT DILUTION APPARATUS PERFORMED IN A THIN FILM SAMPLE

摘要

A method and apparatus for measuring antibody titers in a thin film sample in an automated system which does not require multiple dilutions. The system provides a simple method for creating an in-situ dilution within a sample analysis chamber without the use of any precision fluid-handling components, and further, to use the same principles to provide a wide range of sample dilutions within the chamber so as to obviate the need for additional dilution steps when dealing with samples possibly containing wide ranges of analyte concentrations.