GRADIENT-ELUTION MULTI-COLUMN SEPARATION METHOD

摘要

The invention relates to a method of separating fractions of a mixture to be separated, comprising the following step: provision of a device comprising at least two chromatography columns connected in series; discontinuous injection of the mixture to be separated, at a single point in the device; discontinuous withdrawal of the fraction enriched with the product of interest, at a single point in the device; and injection of eluants into each column and displacement of the eluant injection points, the eluants having different eluting strengths. The method allows effective and easily implementable separation.