摘要 |
Provided is a method of predicting pig litter size by evaluating semen, and more particularly, a method of predicting pig litter size using an in-vitro sperm penetration assay (SPA). This is a novel method of predicting in vivo fertilization using a sperm penetration assay in vitro after optimizing each step of the procedure. Thus, it can more accurately predict excellent individuals for producing smaller or larger litters.
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