Laser-scanning microscope controlling method, involves identifying pixel that exhibits two adjacent empty pixels as intoxication pixels with counting rate of zero, and equating counting rate of intoxication pixels to zero

摘要

The method involves receiving sample light counting rates for multiple pixels by a photon-counting detector (31) e.g. avalanche photodiode, under optical over-sampling. A pixel is identified with a counting rate not equal to zero, where the pixel exhibits two adjacent empty pixels as intoxication pixels with a counting rate of zero. The counting rate of the intoxication pixels is equated to zero, where the intoxication pixels lie in a rapid scanning direction of a laser-scanning-microscope (1). An independent claim is also included for a computer program comprising a set of instructions for performing a method for controlling a laser-scanning microscope.