摘要 |
PROBLEM TO BE SOLVED: To provide a method of detecting a target substance whereby the target substance can be more quickly, accurately and easily detected at a low cost by using a binding substance having been labeled with an oligonucleic acid chain. SOLUTION: The method of detecting a target substance characterized by comprising: the step of contacting a binding substance, which has been labeled with an oligonucleic acid chain having a region capable of binding to a primer to be used in a nucleic acid amplification method available under thermostatic conditions, with the target substance in a test sample to thereby form a complex of the target substance with the binding substance; the step of amplifying the oligonucleic acid chain in the complex by the nucleic acid amplification method as described above; and the step of detecting the amplification product. COPYRIGHT: (C)2010,JPO&INPIT
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