SERUM COLLECTION METHOD FOR DIAGNOSTICS OF ANAPLASMOSIS OF CATTLE IN REACTION OF INDIRECT IMMUNOFLUORESCENCE BY MEANS OF HYPERIMMUNISATION OF RABBITS

摘要

FIELD: veterinary science. ^ SUBSTANCE: method pertains to laboratory diagnostic methods of anaplasmosis and may be used in veterinary. Method consists in injecting of antigen and levamisole immunostimulant to the rabbits. Whereat as an antigen an antigen derived by alternating freezing and thawing of the grown on the Vero cells in the growth supporting nutrient medium "Igla" MEM with added embryonic serum, amino acids and antibiotics of vial culture Anaplasma sp. Omsk after the eight days of incubation. ^ EFFECT: method enables getting anaplasmosis serum with high antibodies diagnostic titer. ^ 5 tbl