| 摘要 |
Disclosed is a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ornithine decarboxylase activity, compared with the native level of ornithine decarboxylase activity of the microorganism is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, compared with the translational and/or transcriptional efficiency with the native level of ornithine decarboxylase activity of the microorganism, and that 1,4-bunanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth, wherein: the increased translational and/or transcriptional efficiency is obtained by the use of a strong, regulated promoter selected from the group consisting of an isopropyl-beta-D-thiogalactoside (IPTG) inducible strong promoter, T7, T5, ptac, and plac promoters. |
