METHOD AND KIT FOR DETECTION/IDENTIFICATION OF VIRUS-INFECTED CELL
摘要
<p>Disclosed is a means for specifically detecting and identifying a virus-infected cell in a floating cell system by a simple manipulation and with high sensitivity. A virus-infected cell can be detected and identified by the following steps (1) to (5): (1) adding a first labeled antibody which has been labeled with a first labeling substance and is directed against a cell surface antigen specific to a target cell to a sample and allowing the mixture to react; (2) immobilizing a protein in the presence of an RNA-stabilizing agent; (3) treating the protein with a surfactant; (4) adding a labeled nucleic acid probe for a nucleic acid specific to a target virus to cause the hybridization; and (5) detecting a cell labeled with both the first labeled antibody and the labeled nucleic acid probe by flow cytometry.</p>
申请公布号
WO2009116266(A1)
申请公布日期
2009.09.24
申请号
WO2009JP01173
申请日期
2009.03.17
申请人
NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY;KIMURA, HIROSHI;NISHIYAMA, YUKIHIRO