发明名称 |
PURIFICATION OF A BIVALENTLY ACTIVE ANTIBODY USING A NON-CHROMATOGRAPHIC METHOD |
摘要 |
The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment.
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申请公布号 |
US2009240040(A1) |
申请公布日期 |
2009.09.24 |
申请号 |
US20070303089 |
申请日期 |
2007.06.01 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
KRISHNAMURTHY VIJAY M.;ESTROFF LARA A.;SEMETEY VINCENT;THOMAS SAMUEL W.;KAUFMAN GEORGE K.;BILGICER ZIHNI BASAR;WHITESIDES GEORGE M. |
分类号 |
C07K1/00 |
主分类号 |
C07K1/00 |
代理机构 |
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代理人 |
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地址 |
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