发明名称 |
Novel sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant |
摘要 |
It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling.
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申请公布号 |
US2009240032(A1) |
申请公布日期 |
2009.09.24 |
申请号 |
US20060920878 |
申请日期 |
2006.05.26 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY;NATIONAL UNIVERSITY CORPORATION;YASUO SUDA |
发明人 |
SUDA YASUO;NISHIMURA TOMOAKI;KISHIMOTO YUKO;NAKAGAWA HIROMI |
分类号 |
G01N33/68;C07H1/00;C07K1/00 |
主分类号 |
G01N33/68 |
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