发明名称 |
A METHOD FOR PREPARING ENDODERMAL PRECURSOR CELLS AND INSULIN SECRETION CELLS FROM WOMEN LABIA MINORA SKIN CELL BY REPROGRAMMING METHOD, AND USE OF REPROGRAMMED INSULIN SECRETION CELLS AS A CELL THERAPY PRODUCT |
摘要 |
PURPOSE: A method for preparing insulin-secreting cells through reprogramming from labium minor-derived adult cells is provided to easily and rapidly obtain massive adult cells from labium minor. CONSTITUTION: A method for inducing the differentiation of pluripotent adult stem cells which are derived from labium minor to insulin-secreting cells comprises: a step of culturing the adult stem cells in the media containing low glucose DMEM, 10% of FBS and 1% L-glutamine and 1% of penicillin-streptomycin; a step of culturing cultured adult stem cell in ITS media containing DMEM/F12(1:1), 5μg/ml of insulin, 50ug/ml of transferrine, 30nM of selenium and 1mM of glutamine; and a step of culturing the cultured cells in CM media to induce the differentiation. The CM media contains 10% of FBS (fetal bovine serum), 1% of L-glutamine (cambrex), 1% of penicillin-streptomycin, 0.5μM of hydrocortisone, 5μg/ml of insulin and 10 mM of nicotinamide. |
申请公布号 |
KR20090100899(A) |
申请公布日期 |
2009.09.24 |
申请号 |
KR20080026418 |
申请日期 |
2008.03.21 |
申请人 |
STEMMEDIENCE CO., LTD. |
发明人 |
YOU, SEUNG KWON;KIM, BO NA;YOON, BYUNG SUN;MOON, JAI HEE;JUNG, HYE YOUN;MAENG, ISAAC;JUN, EUN KYOUNG;PARK, GYU MAN |
分类号 |
C12N5/071;C12N5/08;C12N5/00;C12N5/02 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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