Quantification of analytes using internal standards

摘要

The present invention pertains to methods of quantifying the levels of at least one analyte in a sample or extract comprising adding a known quantity of at least one internal standard to the sample or extract. The present invention also relates to internal standards used in mass spectrometry, as well as compositions thereof. Internal standards for mass spectrometry according to the invention can be used, for example, to assist aligning mass spectra obtained from two different samples, each of which comprises the internal standard. In one aspect of the invention, the internal standard is a dendrimer. A labile internal standard may be used in conjunction with the dendrimer.