METHOD AND DEVICE FOR THE THERMAL CONTROL OF TEMPERATURE-DEPENDENT ENZYMATIC REACTIONS USING MAGNETIC PARTICLES OR MAGNETIC BEADS AND ALTERNATING MAGNETIC FIELDS

摘要

The invention relates to a method for the thermal control of at least one temperature-dependent enzymatic reaction in the presence of magnetic particles, particularly nanoparticles, or magnetic beads, in vitro by heating the magnetic beads or magnetic particles to at least one defined target temperature using alternating magnetic fields. The thermally controllable enzymatic reaction carried out with the method according to the invention is preferably a PCR reaction or another reaction for elongating or multiplying nucleic acids, including DNA, RNA, or hybrids or derivatives thereof, which takes place directly on the functionalized magnetic beads. Further aspects of the invention relate to a reactor for carrying out the method and to the use of the method or the reactor in analytics and diagnostics.