| 摘要 |
<p>Provided are a complex of lactoferrin with a hydrophilic, linear and non-peptidic polymer which has a reduced antigenicity, imparts a pepsin-resistance, shows a prolonged life in vivo and, therefore, has a high clinical usefulness, and a method of producing the same. Also provided are a lactoferrin complex which sustains the biological activity of natural lactoferrin at a definite ratio, shows a significantly prolonged life in vivo and is superior in clinical usefulness to natural lactoferrin, a method of producing the same and so on. A method of producing a polyethylene glycolated lactoferrin complex wherein linear polyethylene glycol (PEG) or a modified product thereof is covalently bonded to lactoferrin via an amide bond, characterized by comprising a step of reacting a liquid reaction mixture, which contains lactoferrin and a linear PEG derivative having a para-nitrophenyl group, under such conditions as allowing the formation of an amide group between the para-nitrophenyl group and lactoferrin as described above.</p> |
