发明名称 |
ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS |
摘要 |
An inventive substrate is provided which includes a substrate compound of formula AB1B2B3B4: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B1B2B3B4, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity. |
申请公布号 |
CA2718276(A1) |
申请公布日期 |
2009.09.17 |
申请号 |
CA20092718276 |
申请日期 |
2009.03.13 |
申请人 |
PERKINELMER LAS, INC. |
发明人 |
CERDA, BLAS;BOBROW, MARK NORMAN |
分类号 |
C07K19/00;A61K31/7042;C12Q1/06;C12Q1/54 |
主分类号 |
C07K19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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