| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a luciferase using 2-(1-methylpropyl)-6-(naphthalen-2-yl)-8-(3-guanidinopropyl)imidazo[1,2-a]pyrazin-3-one as a substrate and improved in luminescence intensity. <P>SOLUTION: A mutant luciferase is derived from Cypridina noctiluca and composed of an amino acid sequence in which specific amino acid residues are replaced and has a luminescence intensity which is at least 1.4 times that of wild type luciferase when using 2-(1-methylpropyl)-6-(naphthalen-2-yl)-8-(3-guanidinopropyl)imidazo[1,2-a]pyrazin-3-one as a substrate. <P>COPYRIGHT: (C)2009,JPO&INPIT |
