Beta-fructofuranosidase and its gene, method of isolating beta-fructofuranosidase gene, system for producing beta-fructofuranosidase, and beta-fructofuranosidase variant

摘要

A novel ² -fructofuranosidase gene and a ² -fructofuranosidase encoded by the gene, a process for isolating a ² -fructofuranosidase gene using the novel ² -fructofuranosidase gene, and a novel ² -fructofuranosidase obtained by this isolation process are disclosed. A novel mold fungus having no ² -fructofuranosidase activity suitable for the production of ² -fructofuranosidase, and a system for producing a recombinant ² -fructofuranosidase using the novel mold fungus as a host is disclosed. Further, a ² -fructofuranosidase variant which selectively and efficiently produces a specific fructooligosaccharide such as 1-kestose from sucrose is disclosed.