METHODS FOR SCREENING INITIAL CODONS PROVIDING DESIRED EXPRESSION LEVELS OF PROTEINS, AND METHODS FOR TUNING EXPRESSION AND PRODUCTION OF RECOMBINANT PROTEINS

摘要

A method for screening an initial codon, which provides target protein level is provided to express an active protein at high speed and synthesize signal peptide having expression intensity by regulating the expression intensity of signal peptide. A method for screening an initial codon, which provides expression level of target protein comprises: a step of changing the target gene in one or more site of +2 to +13 codon in random; a step of isolating individual gene and amplifying the isolated gene through colony PCR; a step of performing cell-free protein synthesis; and a step of selecting a codon by irradiating.