pH dependent ion exchange material capable of selectively binding to nucleic acids in comparison with proteins, solid substrate having the material immobilized on its surface, and method of isolating a nucleic acid using the material or the solid substrate

摘要

Provided is a pH dependent ion exchange material. The pH dependent ion exchange material has at least one monomer selected from the group consisting of M0, M1, M2 and M3 represented by the following formulae, provided that at least one monomer having A and at least one monomer having B are contained therein, the pH dependent ion exchange material having a degree of polymerization of 2-30,000. wherein A is a group -X(CH2)nY, wherein n is an integer from 1 to 10, X is a functional group which can react with an activated ester, and Y is a primary, secondary, tertiary amine or a nitrogen-containing aromatic heterocyclic base, B is a group -X'(CH2)nY', wherein n is an integer from 1 to 10, X' is a functional group which can react with an activated ester, and Y' is an acid generating group having a pKa value of 4 or less, and R3 is an alkyl group having 1 to 10 carbon atoms.