METHODS AND COMPOSITIONS FOR REDUCING NUCLEOTIDE IMPURITIES

摘要

The disclosure provides methods and compositions for reducing nucleotide impurities in reagents and reaction mixtures. Generally, the methods of the invention involve the inclusion of so-called 'scrubbing oligonucleotides' (or 'scrubbers') that preferentially incorporate nucleotide impurities, thereby reducing available free impurities. The disclosure further provides methods of sequencing a target nucleic acid by synthesis that utilize 'live' scrubbing. Scrubbing oligonucleotides of various structures are disclosed, including hairpin scrubbers and homopolymeric scrubbers.