发明名称 |
SHRNA-MEDIATED INHIBITION OF EXPRESSION OF ALPHA-1. 6-FUCOSYLTRANSFERASE |
摘要 |
<p>The current invention comprises a method for producing a heterologous polypeptide with a reduced degree of fucose modification in a mammalian cell by cultivating the mammalian cell under conditions suitable for the expression of said heterologous polypeptide, and recovering the heterologous polypeptide from the mammalian cell or the culture, wherein in said mammalian cell the enzymatic activity of alpha1,6-fucosyltransferase is reduced by means of an shRNA directed against alpha1,6-fucosyltransferase mRNA.</p> |
申请公布号 |
EP2097445(A1) |
申请公布日期 |
2009.09.09 |
申请号 |
EP20070856883 |
申请日期 |
2007.12.19 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
BEUGER, VINCENT;BURTSCHER, HELMUT;KLEIN, CHRISTIAN |
分类号 |
C07K16/00;C12N5/10;C12N9/10;C12N15/113;C12N15/85 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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