| 摘要 |
A method for detecting and monitoring a condition in a subject using a single molecule detector is disclosed comprising detecting a first marker in a first sample from the subject, wherein the first marker comprises a biomarker such as Cardiac Troponin-I (cTnI), and wherein the limit of detection of the first marker is less than 20 pg/ml, and further comprising the detection of a second marker as a further indication of the condition to be detected. The second marker can be a biomarker, physiological marker, a molecular marker or a genetic marker. Also claimed are similar methods involving the detection of Vascular Endothelial Growth Factor (VEGF), pro B-type Natriuretic Peptide (proBNP), TREM-1, Interleukin 1 Alpha (IL-1 a ), IL- 1 b , IL-4, IL-6, IL-8, IL-10, Interferon gamma (IFN- q ), Tumour Necrosis Factor alpha (TNF- a ), Glucagon-like peptide-1 (GLP-1), Leukotriene E4 (LTE4), Transforming Growth Factor Beta (TGF- b ), Akt1, A b -40, A b -42, and Fas ligand (FasL). The markers may be detected using fluorescent labels.
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