A METHOD FOR PRODUCING N-TERMINAL METHIOINE FREE POLYPEPTIDES IN MICROBIAL HOST CELLS

摘要

A method of making N-terminal methionine free polypeptide in a recombinant microbial host cell and pharmaceutical compositions along with one or more pharmaceutical excipients for management of various therapeutic disorders. Specifically, the invention relates to the production of N-terminal methionine free Streptokinase or human growth hormone by co expression with a protease enzyme in E.coli.