| 摘要 |
The invention refers to agriculture, particularly to methods for selecting salinization-resistant tomato genotypes.The method, according to the invention, includes collection of completely tripped flowers of each genotype, separation of pollen from anthers, cultivation at the temperature of 27?C, during 3 hours, of one part of control pollen on a nutrient medium, containing, in g/L:Saccharose�145...155boric acid��0.5...0.7distilled water�the rest,and of another part of sample pollen on a nutrient medium, containing, in g/L:saccharose�145...155boric acid��0.5...0..sodium chloride�0,245...0.255distilled water�the rest,determination of sample and control pollen viability by the ratio between the number of germinated pollen grains and the total number of analyzed grains, expressed in percents; determination of pollen tubes length by means of the ocular micrometer; calculation of pollen resistance indexes by the ratio of sample pollen viability about the control one, expressed in percents; and of pollen tubes resistance indexes by the ratio of sample pollen tubes length about the control ones, expressed in percents; and calculation of mean integral resistance of genotypes to salinization, expressed by the half-sum of pollen and pollen tubes resistance indexes, at the same time there are selected genotypes, to which the salinization resistance indexes are greater.
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