Polyvalent viral vectors and a system for production thereof

摘要

Provided is a plasmid comprising adenoviral genomic nucleotide sequences to be packaged into a polyvalent adenoviral capsids and: (a) a first movable cassette located in a first locus of an adenoviral genomic sequence, said first movable cassette comprising nucleic acid sequences comprising a first colorimetrically detectable reporter gene operably linked to sequences that will direct expression thereof, said movable cassette being flanked by a first set of rare restriction enzyme sites composed of a rare restriction enzyme site at the 5' end of the first movable cassette and a rare restriction enzyme site at the 3' end of the first movable cassette; and (b) a second movable cassette located in a second locus of the adenoviral genomic sequence which is non-contiguous with the first locus, said second movable cassette comprising nucleic acid sequences comprising a second colorimetrically detectable reporter gene operably linked to sequences that will direct expression thereof, said movable cassette being flanked by a second set of rare restriction enzyme sites composed of a rare restriction enzyme site at the 5' end of the first movable cassette and a rare restriction enzyme site at the 3' end of the second movable cassette; wherein the product of said first detectable reporter gene and the second detectable reporter gene are distinguishable from one another by colour, and wherein the first and second set of rare restriction enzyme sites differ, said rare restriction enzyme sites being such that a corresponding rare restriction enzyme cuts these sites and neither the genetic element carrying the viral genome nor other locations in the viral genome are cut by the enzyme.