发明名称 |
Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production |
摘要 |
Provided is a method for making an immunoprotective particle or a biologically active protein particle comprising the steps of: a) transforming a plant cell with a polynucleotide encoding at least one immunoprotective antigen or at least one biologically active protein; b) culturing said transformed plant cell under conditions that allow for the proliferation of said transformed plant cell and the accumulation of said immunoprotective antigen or said biologically active protein in said plant cell; c) collecting and washing said cultured transformed cells; d) resuspending said washed transformed cells in a lysis buffer containing no detergents or other chemical agents that disrupt cells; e) physically or mechanically disrupting said resuspended cells such that immunoprotective particles or biologically active protein particles are formed; f) separating cellular debris from said immunoprotective particles or said biologically active protein particles to form a solution comprising said immunoprotective particles or said biologically active particles; and (g) filtering said solution comprising immunoprotective particles or said biologically active particles to remove any bacterial contaminants. Further provided are compositions made by the method and various uses of the compositions.
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申请公布号 |
NZ542933(A) |
申请公布日期 |
2009.08.28 |
申请号 |
NZ20040542933 |
申请日期 |
2004.05.04 |
申请人 |
DOW AGROSCIENCES LLC |
发明人 |
MILLER, TIMOTHY J;FANTON, MATTHEW JAMES;WEBB, STEVEN ROBERT |
分类号 |
A01H15/00;A01H1/00;A61K;C12N7/02;C12N15/82 |
主分类号 |
A01H15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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