发明名称 |
Method for transformation using mutant acetolactate synthase gene |
摘要 |
Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.
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申请公布号 |
US2009217420(A1) |
申请公布日期 |
2009.08.27 |
申请号 |
US20060920161 |
申请日期 |
2006.05.09 |
申请人 |
TORIYAMA KINYA;OKUZAKI AYAKO;KAKU KOICHIRO;KAWAI KIYOSHI;SHIMIZU TSUTOMU |
发明人 |
TORIYAMA KINYA;OKUZAKI AYAKO;KAKU KOICHIRO;KAWAI KIYOSHI;SHIMIZU TSUTOMU |
分类号 |
A01H1/00;C12N15/87 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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