| 摘要 |
PROBLEM TO BE SOLVED: To simplify an entire process of reverse transcription-polymerase chain reaction (RT-PCR) by using oligonucleotide-immobilized microplates made of polypropylene, etc. SOLUTION: Oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. The RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted on the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication. COPYRIGHT: (C)2009,JPO&INPIT
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