| 摘要 |
PROBLEM TO BE SOLVED: To provide a fluorescent labeled oligonucleotide for nucleic acid detection to enable easy quantitative analysis of a gene with excellent analytic efficiency without necessitating the preparation of a calibration curve and to provide a method for getting information on double strand formation by using the fluorescent labeled oligonucleotide for nucleic acid detection. SOLUTION: Provided is a fluorescent labeled oligonucleotide P<SB>1</SB>for nucleic acid detection having a base sequence complementary to a nucleic acid strand T for detection, wherein the fluorescent characteristic varies by the double strand formation with the nucleic acid strand T for detection. The fluorescent labeled oligonucleotide P<SB>1</SB>for nucleic acid detection can be produced by labeling with an intercalating fluorescent dye 1 and a non-intercalating fluorescent dye 2. Also provided is a method for getting information on the double strand formation by using the fluorescent labeled oligonucleotide P<SB>1</SB>for nucleic acid detection. COPYRIGHT: (C)2009,JPO&INPIT
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