LARGE-SCALE PRODUCTION OF B-GLUCAN THROUGH SEMI-CONTINUOUS FERMENTATION PERFORMED WITH SPARASSIS CRISPA MYCELIA

摘要

A method for culturing a Sparassis crispa mycelia is provided to massively produce Sparassis crispa mycelia and shorten culture time through semi-continuous culture of liquid phase. A medium for culturing Sparassis crispa mycelia in liquid phase comprises 0.01~3.0% of glucose, 0.01~0.5% of yeast extract, 0.01~0.2% malt extract, 0.01~1% of potassium monophosphate, and 0.01~0.04% of iron sulfate. A method for culturing the 0.01~0.04% in liquid phase comprises: a step of germinating a spore of Sparassis crispa mycelia; a step of culturing in liquid phase medium at 23‹C, 200 rpm of shaking speed for seven days; and a step of collecting beta-glucan from Sparassis crispa mycelia.