<p>A method of detecting the presence of a plurality of respiratory viruses using a multiplexed diagnostic assay is disclosed. The method provides a plurality of oligonucleotides that each is specific for a specific respiratory virus. A multiplex PCR is run using the oligonucleotides, which produces double-stranded products. The method also provides a plurality of extension oligonucleotides that each is specific for a specific double-stranded product. Each extension oligonucleotide also has a distinct second portion having a unique sequence. A primer extension reaction is run using the extension oligonucleotides, which produces single-stranded products. The single-stranded products are hybridized to a solid carrier.</p>
申请公布号
WO2009102369(A2)
申请公布日期
2009.08.20
申请号
WO2008US84244
申请日期
2008.11.20
申请人
AUTOGENOMICS, INC.;CORBEIL, JACQUES;RAYMOND, FREDRIC;BOIVIN, GUY