PROCESS FOR PREPARING UNAGGREGATED ANTIBODY FC DOMAINS
摘要
Methods useful for producing a solution of purified Fc peptide chains are disclosed. The methods employ protein A chromatography to separate high molecular weight, aggregated peptide chains comprising antibody Fc domains from lower molecular weight unaggregated peptide chains that also comprise antibody Fc domains. The solutions of purified Fc peptide chains obtained by the methods of the invention contain less than 5% aggregate and greater than 70% of the Fc peptide chains subjected to purification.
申请公布号
EP2089429(A2)
申请公布日期
2009.08.19
申请号
EP20070854226
申请日期
2007.10.19
申请人
CENTOCOR ORTHO BIOTECH INC.
发明人
ALFONSO, PEDRO;CAPALDI, MICHAEL;DONG, DENNIS;GERVAIS, THOMAS R.;GOLDSTEIN, JOSHUA;MAGILL, ALLEN B.;QUINLAN, NICOLE;SAINI, DEEPAK