CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES

摘要

DNA sequence (I) coding for an Aspergillus fumigatus phospholipase (II) is new. DNA sequence (I) having SEQ ID NO:1 (a defined sequence of 2822 nucleotides given in the specification) and coding for a phospholipase (II) with SEQ ID NO:2 (a defined sequence of 633 amino acids given in the specification) is new. Independent claims are also included for: (1) expression construct comprising (I) linked to regulatory sequences for expression in a host cell; (2) recombinant host cell transformed with the expression construct; (3) plasmid B11B1Hind6 (DSM 18369); (4) phospholipase encoded by the coding portion (especially nucleotides 530-2388) of (I); (5) producing (II) by culturing a host cell as above; (6) desliming vegetable oil by adding an aqueous solution containing (II), heating at 30-70[deg] C for 1-12 hours and separating the aqueous and oil phases.