| 摘要 |
PROBLEM TO BE SOLVED: To easily find analytical conditions in a short time such as an excitation light wavelength and a fluorescence wavelength optimal for highly accurate determination of each sample component separated by a column. SOLUTION: A target sample while being fixed to a prescribed excitation light wavelength and a prescribed fluorescence wavelength is analyzed by liquid chromatography (S1 and S2). When an intensity value takes a threshold value or greater, and its amount of change becomes small, a sample component is considered to have appeared (S3-S6) to obtain a retention time at the time and temporarily halt liquid feeding to the column (S7 and S8). With an eluate containing the sample component retained in a flow cell, an excitation light wavelength and a fluorescence wavelength are each scanned at high speed to obtain a schematic three-dimensional spectrum and store it with the retention time (S9-S10). At each time a sample component separated by the column has appeared, this is repeated to determine a three-dimensional spectrum corresponding to each sample component. A peak top having maximum intensity in the three-dimensional spectrum is searched for to find the excitation light wavelength and the fluorescence wavelength that provide the peak and display them with the retention time. COPYRIGHT: (C)2009,JPO&INPIT
|
