<p>The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75°C to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27°C, preferably between 17.5 and 24.6°C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.</p>
申请公布号
WO2009099375(A1)
申请公布日期
2009.08.13
申请号
WO2009SE00063
申请日期
2009.01.30
申请人
GE HEALTHCARE BIO-SCIENCES AB;GLAD, GUNNAR;JOHANSSON, BO-LENNART;MALOISEL, JEAN-LUC;NORRMAN, NILS;STENHOLM, AAKE