发明名称 |
PROCESS FOR PRODUCING POLYPEPTIDE |
摘要 |
The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.
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申请公布号 |
US2009203078(A1) |
申请公布日期 |
2009.08.13 |
申请号 |
US20090364049 |
申请日期 |
2009.02.02 |
申请人 |
KYOWA HAKKO KOGYO CO., LTD. |
发明人 |
OGAWA TATSUYA;KONNO YOSHINOBU;AKASHI NAOHISA;TAKASUGI HIROSHI;SUGIMOTO SEIJI;YANO KEIICHI |
分类号 |
C12P21/00;C07K16/00;C12N5/06 |
主分类号 |
C12P21/00 |
代理机构 |
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主权项 |
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地址 |
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